1. The Gram negative organism is
A. Actinomyces
B. Bacillus
C. Clostridium
D. None of these
View Answer
Answer: D
2. Catalase production is negative in which of the following?
A. Streptococcus
B. Salmonella
C. Proteus
D. Staphylococcus
View Answer
Answer: A
3. Which of the following is a substitute for crystal violet used in gram-staining procedure?
A. Methylene blue
B. Bromocresol green
C. Safranin
D. Phenolpthalene
View Answer
Ans: A
4. Which of the following inference(s) indicate(s) the ability of an organism to utilize citrate as a sole source of carbon in Simmon’s citrate medium?
A. Blue colour
B. Appearance of growth
C. Both (a) and (b)
D. None of these
View Answer
Ans: A
5. The organisms that can be acid-fast stained is
A. Nocardia
B. Tuhercle bacilli
C. Lepra Bascilli
D. All of these
View Answer
D
6. The primary stain of Gram’s method is
A. safranin
B. phenolpthalene
C. crystal violet
D. methyl red
View Answer
C
7. At what concentration of sulphuric acid, Nocardia resists decolourization?
A. 0.5% sulphuric acid
B. 5% sulphuric acid
C. 10% sulphuric acid
D. 8% sulphuric acid
View Answer
A
8. Which of the following is commonly used as Gram’s decolouriser?
A. Ethyl alcohol
B. Methyl alcohol
C. Acetone
D. A mixture of ethyl alcohol and acetone
View Answer
D
9. Production of acetone can be detected by which of the following tests?
A. Citrate test
B. Voges-Proskauer test
C. Methyl red test
D. Indole test
View Answer
D
10. The production of sufficient acid by fermentation of glucose leads to decrease in pH of the medium falls Below 4.5. Which if the following test can detect it?
A. Indole test
B. Methyl red test
C. Citrate utilization test
D. Voges-Proskauer test
View Answer
B
11. What is the colour of gram -postive bacteria on a gram staining?
A. Purple
B. Pink
C. Colourless
D. Green
View Answer
A
12. What is the colour of gram-negative bacteria on a gram staining?
A. Purple
B. Pink
C. Green
D. Colourless
View Answer
B
13. Which of the following is a primary stain for acid fast staining of mycobacteria?
A. Crystal violet
B. Carbol fuchsin
C. geimsa
D. Methylene blue
View Answer
B
14. The deinococci are gram-positive cocci and rods that are distinctive in their unusually great resistance to
A. radiation
B. desiccation
C. Both (a) and (b)
D.Dextreme temperatures
View Answer
C
15. Which of the following shows a positive urease test?
A. Proteus
B. Klebsiella
C. Both (a) and (b)
D. None of these
View Answer
C
16. Which of the following(s) is/ are oxidase-positive bacteria?
A. Vibrio
B. Neisseria
C. Pseudomonas
D. All of these
View Answer
D
17. What is the order of reagents used in the Gram stain?
A. Crystal violet, iodine, safranin, decolorizer
B. Crystal violet, iodine, decolorizer, safranin
C. Safranin, crystal violet, decolorizer, iodine
D. Crystal violet, safranin, iodine, decolorizer
View Answer
B
18. Gram staining was developed accidentally in 1884 by
A. Alexander Fleming
B. Hans Christian Gram
C. Joseph Christian Gram
D. Robert Gram
View Answer
B
19. Which of the following organisms can be demonstrated with silver impregnation method?
A. Vibrios B
B. Salmonella
C. Spirochaetes
D. Shigella
View Answer
C
20. Which of the following(s) show(s) positive results for phenylalanine deaminase test?
A. Providencia
B. Proteus
C. Morganella
D. All of these
View Answer
D
21. The process of Gram staining is based on the ability of bacterial cell wall
A. to retain the safranin dye
B. to retain the crystal violet dye during solvent treatment.
C. to retain part of both dyes
D. none of above
View Answer
B
22. All of the following bacteria are urease test positive except:
a. Klebsiella sp.
b. yersinia enterocolitica
c. Yersinia pestis
d. Helicobacter pylori
View Answer
C
23. Catalase test is negative in:
a. Staphylococcus aureus
b.Salmonella typhi
c. Streptococcus pyogenes
d. Klebsiella sp.
View Answer
C
24. All of the following bacteria are oxidase test positive except:
a. Neisseria meningitides
b. Vibrio cholera
c. Escherichia coli
d. Pleisiomonas sp.
View Answer
C
25. Phenylalanine deaminase test is negative in:
a. Proteus vulgaris
b. Providencia sp.
c. Morganella sp.
d. Klebsiella sp.
View Answer
D
26. Which of the following tests detects the production of acetyl methyl carbinol from pyruvic acid in the media?
a. Methyl red test
b. Voges-Proskauer test
c. Urease test
d. Citrate utilisation test
View Answer
B
27. Triple sugar iron (TSI) agar medium contains all the following carbohydrates except:
a. Glucose
b. Lactose
c. Sucrose
d. Mannitol
View Answer
D
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