Microbiology QUIZ Part IV – STERILISATION AND DISINFECTION | GPAT, Drug Inspector, DCO, Pharmacist, EXIT, NIPER

/12

Created on August 18, 2022 By

Pharmapedia

Microbiology

Microbiology QUIZ Part IV-STERILISATION AND DISINFECTION

1 / 12

The process by which an article, surface or medium is made free of all microorganisms either in the vegetative spore form is known as:

A) Sterilisation

B) Disinfection

C) Asepsis

D) None of the above

2 / 12

Chemical disinfectants which can be safely applied to living tissues to prevent infection are known as:

A) Antiseptics

B) Formaldehyde

C) Ethylene oxide

D) None of the above

3 / 12

Temperature and time period used in hot air oven is:

A) 160℃ for two hours

B) 140℃ for two hours

C) 160℃ for one hours

D) None of the above

4 / 12

Temperature and time period used in holder method of Pasteurization is

A) 63℃ for 30 minutes

B) 63℃ for 50 minutes

C) 72℃ for 20 seconds

D) 72℃ for 40 seconds

5 / 12

Which of the following bacteria can survive in holder method of Pasteurization?

A) Bordetella pertussis

B) Coxiella burnetii

C) Salmonella typhi

D) Mycobacterium bovis

6 / 12

Stream sterilization at 100 for 20 minutes on three successive days is known as

A) Tyndallisation

B) Inspissation

C) Pasteurisation

D) Vaccine bath

7 / 12

Which of the following sterilization conditions are used in autoclave?

A) 121℃, 15 pounds pressure/sq inch, 15 minutes

B) 160℃, 20 pounds pressure/sq inch, 20 minutes

C) 140℃, 10 pounds pressure/sq inch, 15 minutes

D) None of the above

8 / 12

Which bacterial spores are used as sterilization control in autoclave?

A) Clostridium perfringens

B) Bacillus cereus

C) Bacillus stearothermophilus

D) Pseudomonas aeruginosa

9 / 12

Gamma radiations can be used for sterilization of:

A) Plastic syringes

B) Catheters

C) Swabs

D) All of the above

10 / 12

Glutaraldehyde is used for sterilization of

A) Cystoscopes

B) Endoscopes

C) Bronchoscopes

D) All of the above

11 / 12

The most widely used disinfectant for human immunodeficiency virus (HIV) infected material is

A) Hypochlorite solution

B) Lysol

C) Formaldehyde

D) Mercuric chloride

12 / 12

Which of the following is used commercially for sterilization of disposable plastic items?

A) Ethylene oxide

B) Autoclave

C) Glutaraldehyde

D) Ethyl alcohol

Your score is

The average score is 54%

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Category: Microbiology

/12

Created on August 18, 2022 By

Pharmapedia

Microbiology

Microbiology QUIZ Part IV-STERILISATION AND DISINFECTION

1 / 12

The process by which an article, surface or medium is made free of all microorganisms either in the vegetative spore form is known as:

A) Sterilisation

B) Disinfection

C) Asepsis

D) None of the above

2 / 12

Chemical disinfectants which can be safely applied to living tissues to prevent infection are known as:

A) Antiseptics

B) Formaldehyde

C) Ethylene oxide

D) None of the above

3 / 12

Temperature and time period used in hot air oven is:

A) 160℃ for two hours

B) 140℃ for two hours

C) 160℃ for one hours

D) None of the above

4 / 12

Temperature and time period used in holder method of Pasteurization is

A) 63℃ for 30 minutes

B) 63℃ for 50 minutes

C) 72℃ for 20 seconds

D) 72℃ for 40 seconds

5 / 12

Which of the following bacteria can survive in holder method of Pasteurization?

A) Bordetella pertussis

B) Coxiella burnetii

C) Salmonella typhi

D) Mycobacterium bovis

6 / 12

Stream sterilization at 100 for 20 minutes on three successive days is known as

A) Tyndallisation

B) Inspissation

C) Pasteurisation

D) Vaccine bath

7 / 12

Which of the following sterilization conditions are used in autoclave?

A) 121℃, 15 pounds pressure/sq inch, 15 minutes

B) 160℃, 20 pounds pressure/sq inch, 20 minutes

C) 140℃, 10 pounds pressure/sq inch, 15 minutes

D) None of the above

8 / 12

Which bacterial spores are used as sterilization control in autoclave?

A) Clostridium perfringens

B) Bacillus cereus

C) Bacillus stearothermophilus

D) Pseudomonas aeruginosa

9 / 12

Gamma radiations can be used for sterilization of:

A) Plastic syringes

B) Catheters

C) Swabs

D) All of the above

10 / 12

Glutaraldehyde is used for sterilization of

A) Cystoscopes

B) Endoscopes

C) Bronchoscopes

D) All of the above

11 / 12

The most widely used disinfectant for human immunodeficiency virus (HIV) infected material is

A) Hypochlorite solution

B) Lysol

C) Formaldehyde

D) Mercuric chloride

12 / 12

Which of the following is used commercially for sterilization of disposable plastic items?

A) Ethylene oxide

B) Autoclave

C) Glutaraldehyde

D) Ethyl alcohol

Your score is

The average score is 54%

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123

Created on August 25, 2022 By

Pharmapedia

Microbiology

Microbiology QUIZ Part X– IMMUNITY & VACCINE

NameEmailMob Number

1 / 14

Which of the following immunity is called the first line of defence?

a) Innate Immunity

b) Active immunity

c) Passive immunity

d) Acquired immunity

2 / 14

Cells Involved In Innate Immunity are

a) Phagocytes

b) Natural Killer Cells

c) Macrophages

d) All of the above

3 / 14

Skin, body hair, cilia, eyelashes, the respiratory tract and the gastrointestinal tract are examples of

a) Physical barrier

b) Cellular barriers

c) Cytokine barriers

d) Physiological barriers

4 / 14

Which of the following cells is involved in humoral immunity?

a) T-cells

b) B-cells

c) B-cells

d) Both T and B cells

5 / 14

Which of the following conveys the longest-lasting immunity to an infectious agent?

a) Active immunity

b) Passive immunity

c) Both (a) and (b)

d) None of the above

6 / 14

Which of the following cells is involved in cell-mediated immunity?

a) T-cells

b) B-cells

c) Mast cells

d) Both T and B cells

7 / 14

Which of the following statements is true about Passive Immunity?

a) This immunity causes reactions

b) This immunity develops immediately

c) This immunity lasts only for a few weeks or months

d) All of the above.

8 / 14

B-cells and T-cells are two types of cells involved in

a) Innate Immunity

b) Active immunity

c) Passive immunity

d) Acquired immunity

9 / 14

Which of the following immunity is present from our birth?

a) Innate Immunity

b) Active immunity

c) Passive immunity

d) Acquired immunity

10 / 14

All of the following are killed vaccines except

a) Salk vaccine

b) Hepatitis B vaccine

c) Non-neural vaccines for rabies

d) MMR

11 / 14

All of the following are live vaccines except:

a) BCG

b) Sabin vaccine

c) MMR

d) TAB vaccine

12 / 14

Artificial passive immunity may be induced by

a) Antiserum injection

b) Vaccination

c) Maternal antibodies

d) All of the above

13 / 14

Vaccination induces:

a) Active natural immunity

b) Passive natural immunity

c) Active artificial immunity

d) Passive artificial immunity

14 / 14

Clinical or subclinical infections lead to

a) Active natural immunity

b) Passive natural immunity

c) Active artificial immunity

d) Passive artificial immunity

Your score is

The average score is 36%

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Created on August 14, 2022 By

Pharmapedia

Microbiology

Created on August 19, 2022 By

Pharmapedia

Microbiology

Microbiology QUIZ Part V- CULTURE MEDIA & METHODS

1 / 26

Yeast extract is prepared from

Casein

lean meat

heart muscle

Saccharomyces

2 / 26

Which of the following is not true about peptone.

It is hygroscopic

Obtain from lean meat, heart muscle, casein

Mainly supply nitrogenous material

None of the above.

3 / 26

Which of the following is not a storage media.

Egg saline medium

Dorsets egg medium

Blood agar slants

None

4 / 26

In chocolate agar media the concentration of blood is

5 -10%

1 to 2 %

20 to 30%

0.5 to 1%

5 / 26

A culture medium the exact composition of which is not known was called as

Synthetic media

Complex media

Defined Media

Natural media

6 / 26

Which of the following is a characteristic of beef extract?

Product resulting from the digestion of proteinaceous materials

Aqueous extract of lean beef tissue

Aqueous extract of yeast cells

Complex carbohydrate obtained from certain marine algae

7 / 26

Which of the following is a rich source of B vitamins?

Peptone

Yeast extract

Beef extract

Agar

8 / 26

Agar media can be enriched with blood for the identification of?

Bacteria which can grow in anaerobic conditions

Bacteria which can cause hemolysis

Viruses from other microorganisms

Bacteria which can grow in higher hydrostatic pressures

9 / 26

Culture medium used for growth of fungi is

Nutrient agar media

Sabouraud’s medium

Nutrient broth

Minimal agar medium

10 / 26

In the medium other than nutrients, if any substance is used in excess, that medium is

Indicator medium

Special medium

Enriched medium

Sugar medium

In the medium other than nutrients, if any substance is used in excess, that medium is called as Enriched medium

11 / 26

Which of the following is not true about agar?

Source of nutrient

Long chain Polysaccharide

Melts at 95 to 98 degree Centigrade

Solidies at approx. 40 degree Centigrade

12 / 26

Which of the following is the example of Anaerobic medium

Robertson cooked-meat medium

Nutrient agar

Nutrient broth

Mac-Conkey’s agar

13 / 26

For preparation of semi-solid media agar used in concentration between

1 to 2 %

1.5 to 2.5 %

0.2 to 0.5%

0.05 to 0.009%

14 / 26

What is the concentration of agar in solid media

3 to 4 %

1.5 to 2 %

10 t0 15 %

0.5 to 1%

15 / 26

Example of indicator medium is

Nutrient Agar Medium

Mac-Conkey’s agar Medium

Wilson and Blair Medium

Czapeck-dox medium

16 / 26

Blood agar media is used for the growth of?

Fungi

Fastidious bacteria

Halophiles

Red algae only

17 / 26

The most commonly used solidifying agent Agar is obtained from

Brown algae

Red algae

Green alga

Blue-green algae

18 / 26

Peptone water and nutrient broth are

Enriched media

Enrichment media

Basal media

Differential medium

19 / 26

Blood agar medium is

Enrichment medium

Enriched medium

Selective medium

Differential medium

20 / 26

The solidifying agent used in microbiology laboratories to solidify medium

Cellulose

Peptone

Thioglycollate

Agar

21 / 26

Which of the following bacteria produce saccharolytic reaction in cooked meat broth (CMB)?

Costridium perfrngens

Costridium tetani

Pseudomonas aeruginosa

None of the above

22 / 26

Anaerobic jar is commonly used to grow:

Costridium tetani

Klebsiella sp.

Pseudomonas aeruginosa

None of the above

23 / 26

Which enrichment medium is preferred to grow Vibrio cholerae?

Tetrathionate broth

Selenite F broth

All of the above

24 / 26

When a liquid medium contains a substance that inhibits all unwanted bacteria but stimulates the growth of particular bacteria, it is known as:

Selective medium

Enriched medium

Enrichment medium

None

25 / 26

When a solid medium contains a substance that inhibits all unwanted bacteria but facilitates the isolation of particular bacteria, it is known as:

Selective medium

Enriched medium

Indicator medium

None of the above

26 / 26

All of the following are examples of enriched media except:

Blood agar

Chocolate agar

Loeffler’s serum slope

Bile salt agar

Your score is

The average score is 28%

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Created on August 15, 2022 By

Pharmapedia

Microbiology QUIZ -Part II – MICROSCOPY AND MORPHOLOGY OF BACTERIA

1 / 6

Indian ink staining is used to demonstrate

Cell wall

Bacterial capsule

Bacterial flagella

Bacterial spore

2 / 6

All of the following are spore forming bacteria except

Clostridium botulinum

Bacillus subtilis

Bacillus anthracis

Pseudomonas aeruginosa

3 / 6

Which one of the following bacteria is cell wall deficient?

Escherichia coli

Salmonella typhi

Mycoplasma

Treponema pallidum

4 / 6

A tuft of flagella present at one or both ends of bacterial cell, is known as

Monotrichous

Amphitrichous

Lophotrichous

Peritrichous

5 / 6

Lipopolysaccharide is a major component of cell wall in

Gram positive bacteria

Gram negative bacteria

Fungi

Parasites

6 / 6

Peptidoglycan layer of cell wall is thicker in

Gram positive bacteria

Gram negative bacteria

Fungi

Parasites

Your score is

The average score is 49%

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