Type of Staining Techniques

Table Of Contents

Various type of Staining techniques in Microbiology

S. NO.	Staining technique	Dye	Application
1.	Simple Staining	Methylene blue/ basic Fuchsin	Impart same colors to all bacteria
2.	Negative Staining	India Ink/ Nigrosin	Demonstration of bacterail capsules
3.	Impregnation method	Siver	Demonstration of bacterial flagella & Spirochaetes
4.	Differential Staining
	a. Gram staining	primary stain: crystal violet; Gram’s iodine (mordant); decolorizing agent (ethanol/ acetone); Secondary counterstain:safranin	To find out gram-positive bacteria (resist decolorization & retain the color of primary stain i.ee violet)& gram-negative bacteria (decolorized by acetone/ethanol & therefore take counterstain i.e. safranin & appear Red)
	b. Acid fast staining	Carbol fuchsin; 20 % Sulphuric acid (decolorization); Counterstain-methylene blue	Staining of Mycobacteria (tubercle & lepra bacilli); acid fastness is due to high lipid content Mycolic acid
	c. Alberts stains	Albert II iodine solution	Staining of Corynebacteria diphtheriae

Type of Staining technique for bacteria

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