Microbiology

Differential staining: Gram staining & Acid-fast staining technique

As name indicates these technique imparts different colour to different bacteria or bacterial structure. It allows us to differentiate between different kinds of bacterial cells or different parts of a bacterial cell. Differential stain technique includes the following technique:- 1. Gram staining 2. Acid Fast staining/Zeihl-Neelsen staining 3. Albert’s stains 1. Gram staining The most …

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Differential staining, Gram staining, Acid-fast staining, technique, Microbiology, Pharmacy notes, Pharmapedia, ThePharmapedia

Type of Staining Techniques | Microbiology

Various type of Staining techniques in Microbiology S. NO. Staining technique Dye Application 1. Simple Staining Methylene blue/ basic Fuchsin Impart same colors to all bacteria 2. Negative Staining India Ink/ Nigrosin Demonstration of bacterail capsules 3. Impregnation method Siver Demonstration of bacterial flagella & Spirochaetes 4. Differential Staining a. Gram staining primary stain: crystal …

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Mycobacterium leprae: Types of Leprosy, Pathogenesis & Epidemiology of leprosy

Leprosy is a chronic bacterial disease and it is caused by Mycobacterium leprae. Leprosy was first observed by Hansen in 1868 so also known as Hansen disease. It mainly affects the skin, eyes, nose and peripheral nerves. It is a gram-positive acid-fast bacteria for which 5% percent sulphuric acid is used for the decolorization after …

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Classification of Bacteria on the basis of Nutrition, oxygen requirement, growing temperature & pH

Bacteria are prokaryotic organisms that do not contain chlorophyll. They are unicellular and do not show true branching. They differ from eukaryotes in not having a nuclear membrane, a nucleolus, and cell organelles like mitochondria, golgi apparatus and endoplasmic reticulum. They have a single circular chromosome. For growth and multiplication of bacteria, they require nutrition …

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Classification of bacteria, nutrition, oxygen, growing temperature, pharmapedia, thepharmapedia
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